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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PGLYRP1 Polyclonal antibody

PGLYRP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18046-1-AP

Synonyms

SBBI68, PGRP-S, PGRPS, PGRP S, PGRP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGLYRP1 Polyclonal antibody (18046-1-AP)

    WB analysis using 18046-1-AP

    Rat brain tissue was subjected to SDS PAGE followed by western blot with 18046-1-AP (PGLYRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PGLYRP1 Polyclonal antibody (18046-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18046-1-AP (PGLYRP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PGLYRP1 Polyclonal antibody (18046-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 18046-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18046-1-AP (PGLYRP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inrat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18046-1-AP targets PGLYRP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGLYRP1 fusion protein Ag12690 相同性解析による交差性が予測される生物種
Full Name peptidoglycan recognition protein 1
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight25 kDa
GenBank accession numberBC101845
Gene symbol PGLYRP1
Gene ID (NCBI) 8993
RRIDAB_3085550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peptidoglycan recognition protein 1 (PGLYRP1, also known as PGRP-S) belongs to a family of evolutionary conserved innate immunity proteins, which in mammals also include PGLYRP2, PGLYRP3, and PGLYRP4 (PMID: 17275309; 20418257). PGLYRP1 is highly expressed in polymorphonuclear leukocytes and to a lower extent in many non-immune cells (PMID: 20418257). It recognizes bacterial peptidoglycan and functions in antibacterial immunity and inflammation (PMID: 20418257). PGLYRP1 has been identified as a ligand for TREM-1 (PMID: 25595774).

Protocols

Product Specific Protocols
WB protocol for PGLYRP1 antibody 18046-1-APDownload protocol
IHC protocol for PGLYRP1 antibody 18046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols