- Featured Product
- KD/KO Validated
PGM1 Polyclonal antibody
PGM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15161-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse skin tissue, HEK-293 cells, Jurkat cells, mouse heart tissue, rat heart tissue |
Positive IP detected in | mouse skin tissue |
Positive IHC detected in | human hepatocirrhosis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 13 publications below |
IHC | See 4 publications below |
IF | See 1 publications below |
Product Information
15161-1-AP targets PGM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PGM1 fusion protein Ag7269 相同性解析による交差性が予測される生物種 |
Full Name | phosphoglucomutase 1 |
Calculated molecular weight | 61 kDa |
Observed molecular weight | 61 kDa |
GenBank accession number | BC001756 |
Gene symbol | PGM1 |
Gene ID (NCBI) | 5236 |
RRID | AB_2161415 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PGM1 antibody 15161-1-AP | Download protocol |
IHC protocol for PGM1 antibody 15161-1-AP | Download protocol |
IF protocol for PGM1 antibody 15161-1-AP | Download protocol |
IP protocol for PGM1 antibody 15161-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.
| ||
Brain C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis. | ||
J Hazard Mater Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes. | ||
Cancer Lett Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis. | ||
Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma | ||
iScience MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Patricia (Verified Customer) (02-25-2020) | Works very well.
|