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  • KD/KO Validated

PGM1 Polyclonal antibody

PGM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15161-1-AP

Synonyms

Phosphoglucomutase-1, GSD14, EC:5.4.2.2, CDG1T


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Tested Applications

Positive WB detected inHeLa cells, mouse skin tissue, HEK-293 cells, Jurkat cells, mouse heart tissue, rat heart tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15161-1-AP targets PGM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGM1 fusion protein Ag7269 相同性解析による交差性が予測される生物種
Full Name phosphoglucomutase 1
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC001756
Gene symbol PGM1
Gene ID (NCBI) 5236
RRIDAB_2161415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PGM1 antibody 15161-1-APDownload protocol
IHC protocol for PGM1 antibody 15161-1-APDownload protocol
IF protocol for PGM1 antibody 15161-1-APDownload protocol
IP protocol for PGM1 antibody 15161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Metab

Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.

Authors - Marion Curtis
  • KD Validated
humanWB

Brain

C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.

Authors - Scott P Allen
humanWB

J Hazard Mater

Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.

Authors - Ye Zhu
humanWB

Cancer Lett

Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.

Authors - Xintian Chen
humanWB

Front Cell Dev Biol

Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma

Authors - Wenshuo Zhang
humanWB

iScience

MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.

Authors - Dong-Xue Ding

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Patricia (Verified Customer) (02-25-2020)

Works very well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer