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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGM1 Monoclonal antibody (66105-1-Ig)

    WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PGM1 Monoclonal antibody (66105-1-Ig)

    IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PGM1 Monoclonal antibody (66105-1-Ig)

    IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGM1 Monoclonal antibody (66105-1-Ig)

    IHC staining of human liver cancer using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGM1 Monoclonal antibody (66105-1-Ig)

    IHC staining of human liver cancer using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PGM1 Monoclonal antibody (66105-1-Ig)

    IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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PGM1 Monoclonal antibody, PBS Only

PGM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66105-1-PBS
Clone No.2G5A8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66105-1-PBS targets PGM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

Tested Reactivity human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PGM1 fusion protein Ag7294 相同性解析による交差性が予測される生物種
Full Name phosphoglucomutase 1
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC001756
Gene Symbol PGM1
Gene ID (NCBI) 5236
RRIDAB_2881504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36871
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.