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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGM5 Recombinant antibody (82965-1-RR)

    WB analysis using 82965-1-RR (same clone as 82965-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82965-1-RR (PGM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PGM5 Recombinant antibody (82965-1-RR)

    IHC staining of human stomach using 82965-1-RR (same clone as 82965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PGM5 Recombinant antibody (82965-1-RR)

    IHC staining of human stomach using 82965-1-RR (same clone as 82965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82965-1-RR (PGM5 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using PGM5 Recombinant antibody (82965-1-RR)

    FC experiment of PC-3 using 82965-1-RR (same clone as 82965-1-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human PGM5 (82965-1-RR, Clone:230206C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82965-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82965-1-RR

    Affinity and Kinetic Characterization of 82965-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82965-1-RR against Human PGM5 were performed. The affinity constant is 0.719 nM.

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PGM5 Recombinant antibody, PBS Only

PGM5 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82965-1-PBS
Clone No.230206C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

PGMRP, Aciculin, 230206C7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82965-1-PBS targets PGM5 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PGM5 fusion protein Ag34399 相同性解析による交差性が予測される生物種
Full Name phosphoglucomutase 5
Calculated molecular weight 62 kDa
Observed molecular weight62 kDa
GenBank accession numberNM_021965
Gene Symbol PGM5
Gene ID (NCBI) 5239
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15124
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Phosphoglucomutase-like protein 5 (also known as aciculin) is an enzyme encoded by the PGM5 gene. Gene functional studies show that PGM5 is similar to PGM1 but lacks enzymatic activity. PGM5 is tightly associated with the actin cytoskeleton and has primarily been investigated as an adhesion protein. It also functions as a cytoskeletal component of cell-matrix and cell-cell contacts in muscle and non-muscle cells. (PMID: 35819729)