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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PR Polyclonal antibody

PR Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19980-1-AP

Synonyms

NR3C3, PGR, PR, progesterone receptor

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Polyclonal antibody (19980-1-AP)

    IHC staining of human breast cancer using 19980-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PR Polyclonal antibody (19980-1-AP)

    IF Staining of MCF-7 using 19980-1-AP

    Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PR Polyclonal antibody (19980-1-AP)

    IF Staining of MCF-7 using 19980-1-AP

    Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19980-1-AP targets PR in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name progesterone receptor
Calculated molecular weight 933 aa, 99 kDa
GenBank accession numberNM_000926
Gene symbol Progesterone Receptor
Gene ID (NCBI) 5241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.