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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PGRMC2 Polyclonal antibody

PGRMC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24575-1-AP

Synonyms

PMBP, DG6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGRMC2 Polyclonal antibody (24575-1-AP)

    WB analysis using 24575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24575-1-AP (PGRMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PGRMC2 Polyclonal antibody (24575-1-AP)

    WB analysis using 24575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24575-1-AP (PGRMC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PGRMC2 Polyclonal antibody (24575-1-AP)

    IHC staining of human placenta using 24575-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24575-1-AP (PGRMC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PGRMC2 Polyclonal antibody (24575-1-AP)

    IHC staining of human placenta using 24575-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24575-1-AP (PGRMC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PGRMC2 Polyclonal antibody (24575-1-AP)

    IF Staining of HeLa using 24575-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGRMC2 antibody (24575-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, SKOV-3 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24575-1-AP targets PGRMC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGRMC2 fusion protein Ag20077 相同性解析による交差性が予測される生物種
Full Name progesterone receptor membrane component 2
Calculated molecular weight 223 aa, 24 kDa
Observed molecular weight22 kDa, 28 kDa
GenBank accession numberBC016692
Gene symbol PGRMC2
Gene ID (NCBI) 10424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 is highly homologous to PGRMC1. PGRMC2 might play a role in cancer.

Protocols

Product Specific Protocols
WB protocol for PGRMC2 antibody 24575-1-APDownload protocol
IHC protocol for PGRMC2 antibody 24575-1-APDownload protocol
IF protocol for PGRMC2 antibody 24575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols