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  • Featured Product
  • KD/KO Validated

PGRMC2 Monoclonal antibody, PBS Only

PGRMC2 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F10D7

Cat no : 60249-1-PBS

Synonyms

DG6, PGRMC2, PMBP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGRMC2 Monoclonal antibody (60249-1-Ig)

    WB analysis using 60249-1-Ig (same clone as 60249-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60249-1-Ig (PGRMC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PGRMC2 Monoclonal antibody (60249-1-Ig)

    IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60249-1-Ig (PGRMC2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PGRMC2 Monoclonal antibody (60249-1-Ig)

    IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60249-1-Ig (PGRMC2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using PGRMC2 Monoclonal antibody (60249-1-Ig)

    IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using PGRMC2 antibody (60249-1-Ig, Clone: 5F10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using PGRMC2 Monoclonal antibody (60249-1-Ig)

    IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using PGRMC2 antibody (60249-1-Ig, Clone: 5F10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PGRMC2 Monoclonal antibody (60249-1-Ig)

    FC experiment of HepG2 using 60249-1-Ig (same clone as 60249-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PGRMC2 (60249-1-Ig, Clone:5F10D7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60249-1-PBS targets PGRMC2 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PGRMC2 fusion protein Ag20077 相同性解析による交差性が予測される生物種
Full Name progesterone receptor membrane component 2
Calculated molecular weight 223 aa, 24 kDa
Observed molecular weight 24+28 kDa
GenBank accession numberBC016692
Gene symbol PGRMC2
Gene ID (NCBI) 10424
RRIDAB_2881370
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).