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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGT Polyclonal antibody (30434-1-AP)

    WB analysis using 30434-1-AP (same clone as 30434-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30434-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30434-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PGT Polyclonal antibody (30434-1-AP)

    IHC staining of human stomach cancer using 30434-1-AP (same clone as 30434-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30434-1-AP (PGT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30434-1-PBS in a different storage buffer formulation.

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PGT Polyclonal antibody, PBS Only

PGT Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30434-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30434-1-PBS targets PGT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGT fusion protein Ag32275 相同性解析による交差性が予測される生物種
Full Name solute carrier organic anion transporter family, member 2A1
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC041140
Gene Symbol PGT
Gene ID (NCBI) 6578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92959
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (PMID: 8787677,22331663).