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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHACTR3 Polyclonal antibody

PHACTR3 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 18107-1-AP

Synonyms

Scapinin, SCAPIN1, Scaffold-associated PP1-inhibiting protein, H17739, C20orf101

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HL-60 cells using PHACTR3 Polyclonal antibody (18107-1-AP)

    IP experiment of HL-60 using 18107-1-AP

    IP result of anti-PHACTR3 (IP:18107-1-AP, 4ug; Detection:18107-1-AP 1:1500) with HL-60 cells lysate 2240 ug.

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Tested Applications

Positive IP detected inHL-60 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18107-1-AP targets PHACTR3 in IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHACTR3 fusion protein Ag12486 相同性解析による交差性が予測される生物種
Full Name phosphatase and actin regulator 3
Calculated molecular weight 559 aa, 63 kDa
Observed molecular weight60 kDa and 75 kDa
GenBank accession numberBC108303
Gene symbol PHACTR3
Gene ID (NCBI) 116154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PHACTR3 was originally named as the nuclear scaffold-associated PP1-inhibiting protein (Scapinin), which is found in the nuclear insoluble fraction of the leukemia cell line HL-60. However, PHACTR3 is distributed to the plasma membrane in adherent cells, and it enhances cell migration (PMID: 25405772). In humans, there are two PHACTR3 isoforms that are 75 kDa and 60 kDa, respectively (PMID: 32423795).

Protocols

Product Specific Protocols
IP protocol for PHACTR3 antibody 18107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols