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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHACTR3 Polyclonal antibody

PHACTR3 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 18107-1-AP

Synonyms

Scapinin, SCAPIN1, Scaffold-associated PP1-inhibiting protein, H17739, C20orf101

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HL-60 cells using PHACTR3 Polyclonal antibody (18107-1-AP)

    IP experiment of HL-60 using 18107-1-AP

    IP result of anti-PHACTR3 (IP:18107-1-AP, 4ug; Detection:18107-1-AP 1:1500) with HL-60 cells lysate 2240 ug.

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Tested Applications

Positive IP detected inHL-60 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18107-1-AP targets PHACTR3 in IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHACTR3 fusion protein Ag12486 相同性解析による交差性が予測される生物種
Full Name phosphatase and actin regulator 3
Calculated molecular weight 559 aa, 63 kDa
Observed molecular weight60 kDa and 75 kDa
GenBank accession numberBC108303
Gene symbol PHACTR3
Gene ID (NCBI) 116154
RRIDAB_3669291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PHACTR3 was originally named as the nuclear scaffold-associated PP1-inhibiting protein (Scapinin), which is found in the nuclear insoluble fraction of the leukemia cell line HL-60. However, PHACTR3 is distributed to the plasma membrane in adherent cells, and it enhances cell migration (PMID: 25405772). In humans, there are two PHACTR3 isoforms that are 75 kDa and 60 kDa, respectively (PMID: 32423795).

Protocols

Product Specific Protocols
IP protocol for PHACTR3 antibody 18107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols