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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHACTR4 Polyclonal antibody

PHACTR4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13408-1-AP

Synonyms

PRO2963

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse eye tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    WB analysis of mouse eye using 13408-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13408-1-AP (PHACTR4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    WB analysis of human liver using 13408-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13408-1-AP (PHACTR4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PHACTR4 Polyclonal antibody (13408-1-AP)

    WB analysis of A431 using 13408-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13408-1-AP (PHACTR4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PHACTR4 Polyclonal antibody (13408-1-AP)

    IHC staining of human ovary cancer using 13408-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13408-1-AP (PHACTR4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PHACTR4 Polyclonal antibody (13408-1-AP)

    IF Staining of A431 using 13408-1-AP

    Immunofluorescent analysis of A431 cells using 13408-1-AP (PHACTR4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse eye tissue, A431 cells, human liver tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13408-1-AP targets PHACTR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHACTR4 fusion protein Ag4054 相同性解析による交差性が予測される生物種
Full Name phosphatase and actin regulator 4
Calculated molecular weight 702 aa, 78 kDa
Observed molecular weight 71-78 kDa
GenBank accession numberBC029266
Gene symbol PHACTR4
Gene ID (NCBI) 65979
RRIDAB_10638316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHACTR4 antibody 13408-1-APDownload protocol
IHC protocol for PHACTR4 antibody 13408-1-APDownload protocol
IF protocol for PHACTR4 antibody 13408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Biol Macromol

Targeting Phactr4 to rescue chronic stress-induced depression-like behavior in rats via regulating neuroinflammation and neuroplasticity

Authors - Ye Li
View Article