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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHAX Polyclonal antibody

PHAX Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16481-1-AP

Synonyms

RNUXA

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using PHAX Polyclonal antibody (16481-1-AP)

    WB analysis of HeLa using 16481-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PHAX Polyclonal antibody (16481-1-AP)

    WB analysis of HepG2 using 16481-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PHAX Polyclonal antibody (16481-1-AP)

    WB analysis of HepG2 using 16481-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHAX Polyclonal antibody (16481-1-AP)

    IF Staining of HeLa using 16481-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16481-1-AP (PHAX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16481-1-AP targets PHAX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHAX fusion protein Ag9602 相同性解析による交差性が予測される生物種
Full Name phosphorylated adaptor for RNA export
Calculated molecular weight 394 aa, 44 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC021161
Gene symbol PHAX
Gene ID (NCBI) 51808
RRIDAB_2299663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Small nuclear and small nucleolar RNAs (snRNAs and snoRNAs) are essential components of snRNPs and snoRNPs, and have a critical role in the maturation of, respectively, mRNAs and rRNAs within the nucleus of eukaryotic cells. Complex and specific pathways exist for the assembly of snRNPs and snoRNPs, involving, nucleocytoplasmic transport of snRNAs and intranuclear transport between compartments of snoRNAs. In metazoa, a subset of spliceosomal snRNAs are exported from the nucleus after transcription. This export occurs in a large complex containing a snRNA, the nuclear cap binding complex (CBC), RanGTP, the leucine-rich nuclear export signal receptor CRM1/Xpo1, and the recently identified phosphoprotein PHAX (phosphorylated adaptor for RNA export). PHAX contains a conserved single-stranded nucleic acid binding domain (RNA_GG_bind domain) with no sequence homology with any other known RNA-binding module [PMID:15574332,11333016, 15574332]

Protocols

Product Specific Protocols
WB protocol for PHAX antibody 16481-1-APDownload protocol
IF protocol for PHAX antibody 16481-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Adv Sci (Weinh)

TRIM24 Cooperates with Ras Mutation to Drive Glioma Progression through snoRNA Recruitment of PHAX and DNA-PKcs

Authors - Chenxin Xu
View Article