- Featured Product
- KD/KO Validated
Prohibitin Polyclonal antibody
Prohibitin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10787-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, human liver tissue, human brain tissue, NIH/3T3 cells, HeLa cells, Raji cells, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat kidney tissue |
Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 19 publications below |
IHC | See 3 publications below |
IF | See 3 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Product Information
10787-1-AP targets Prohibitin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Prohibitin fusion protein Ag1240 相同性解析による交差性が予測される生物種 |
Full Name | prohibitin |
Calculated molecular weight | 30 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC013401 |
Gene symbol | Prohibitin |
Gene ID (NCBI) | 5245 |
RRID | AB_2164476 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Prohibitin antibody 10787-1-AP | Download protocol |
IHC protocol for Prohibitin antibody 10787-1-AP | Download protocol |
IF protocol for Prohibitin antibody 10787-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Autophagy PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis. | ||
Cell Death Differ Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. | ||
Hum Mol Genet Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. | ||
Cell Mol Gastroenterol Hepatol DJ-1 deficiency in hepatocytes improves liver ischemia-reperfusion injury by enhancing mitophagy. | ||
Elife Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sara (Verified Customer) (10-10-2024) | It works well in immunofluorescence using mouse primary lymphocytes. It localises to the mitochondria.
|
FH Tom (Verified Customer) (02-24-2021) | HEK293T whole cell lysate. Membrane was blocked in 5% BSA for 1 hour then incubated overnight at 4 degrees with the primary antibody (1:1000)
|