• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A375 cells using PHC2 Polyclonal antibody (12867-1-AP)

    WB analysis of A375 using 12867-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PHC2 Polyclonal antibody (12867-1-AP)

    WB analysis of A549 using 12867-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using PHC2 Polyclonal antibody (12867-1-AP)

    IP experiment of HL-60 using 12867-1-AP

    IP result of anti-PHC2 (IP:12867-1-AP, 4ug; Detection:12867-1-AP 1:1500) with HL-60 cells lysate 2240 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PHC2 Polyclonal antibody (12867-1-AP)

    IHC staining of human pancreas cancer using 12867-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PHC2 Polyclonal antibody (12867-1-AP)

    IHC staining of human pancreas cancer using 12867-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PHC2 Polyclonal antibody (12867-1-AP)

    IHC staining of mouse kidney using 12867-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PHC2 Polyclonal antibody (12867-1-AP)

    IHC staining of mouse kidney using 12867-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using PHC2 Polyclonal antibody (12867-1-AP)

    IF Staining of A375 using 12867-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12867-1-AP (PHC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PHC2 Polyclonal antibody (12867-1-AP)

    IF Staining of A549 using 12867-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PHC2 antibody (12867-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PHC2 Polyclonal antibody

PHC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12867-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Polyhomeotic-like protein 2, Polyhomeotic like protein 2, PH2, HPH2, EDR2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, A549 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman pancreas cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

12867-1-AP targets PHC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHC2 fusion protein Ag3507 相同性解析による交差性が予測される生物種
Full Name polyhomeotic homolog 2 (Drosophila)
Calculated molecular weight 858 aa, 91 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC028396
Gene Symbol PHC2
Gene ID (NCBI) 1912
RRIDAB_2877890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHC2 antibody 12867-1-APDownload protocol
IHC protocol for PHC2 antibody 12867-1-APDownload protocol
IF protocol for PHC2 antibody 12867-1-APDownload protocol
IP protocol for PHC2 antibody 12867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Mol Cell

Modularity of PRC1 composition and chromatin interaction define condensate properties

Authors - Stefan Niekamp
View Article