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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    WB analysis of mouse brain using 66589-1-Ig (same clone as 66589-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    WB analysis of HEK-293 using 66589-1-Ig (same clone as 66589-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    WB analysis of SH-SY5Y using 66589-1-Ig (same clone as 66589-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    WB analysis of pig brain using 66589-1-Ig (same clone as 66589-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66589-1-Ig (EGLN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    IHC staining of human testis using 66589-1-Ig (same clone as 66589-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    IHC staining of human testis using 66589-1-Ig (same clone as 66589-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    IHC staining of human kidney using 66589-1-Ig (same clone as 66589-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    IHC staining of human kidney using 66589-1-Ig (same clone as 66589-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66589-1-Ig (EGLN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PHD2/EGLN1 Monoclonal antibody (66589-1-Ig)

    IF Staining of HEK-293 using 66589-1-Ig (same clone as 66589-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHD2/EGLN1 antibody (66589-1-Ig, Clone: 1A2F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66589-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PHD2/EGLN1 Monoclonal antibody, PBS Only

PHD2/EGLN1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66589-1-PBS
Clone No.1A2F1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EGLN1, PHD2, EGLN 1, ECYT3, EC:1.14.11.29

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66589-1-PBS targets PHD2/EGLN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name egl nine homolog 1 (C. elegans)
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa, 44 kDa, 36 kDa
GenBank accession numberNM_022051
Gene Symbol PHD2/EGLN1
Gene ID (NCBI) 54583
RRIDAB_2881949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZT9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.