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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PHEX Monoclonal antibody (66934-1-Ig)

    WB analysis of HEK-293 using 66934-1-Ig (same clone as 66934-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using PHEX Monoclonal antibody (66934-1-Ig)

    WB analysis of rat lung using 66934-1-Ig (same clone as 66934-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig lung tissue using PHEX Monoclonal antibody (66934-1-Ig)

    WB analysis of pig lung using 66934-1-Ig (same clone as 66934-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PHEX Monoclonal antibody (66934-1-Ig)

    IHC staining of human lung cancer using 66934-1-Ig (same clone as 66934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66934-1-Ig (PHEX antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

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PHEX Monoclonal antibody, PBS Only

PHEX Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66934-1-PBS
Clone No.1D4B2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig

Applications

WB, IHC, Indirect ELISA

Synonyms

HPDR, HPDR1, HYP, HYP1, PHEX, XLH

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66934-1-PBS targets PHEX in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Pig samples.

Tested Reactivity Human, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PHEX fusion protein Ag20290 相同性解析による交差性が予測される生物種
Full Name phosphate regulating endopeptidase homolog, X-linked
Calculated molecular weight 749 aa, 86 kDa
Observed molecular weight 86-100 kDa
GenBank accession numberBC105059
Gene Symbol PHEX
Gene ID (NCBI) 5251
RRIDAB_2882259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78562
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660).Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).