Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
15032-1-AP targets PHF17 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PHF17 fusion protein Ag4290 相同性解析による交差性が予測される生物種 |
| Full Name | PHD finger protein 17 |
| Calculated molecular weight | 96 kDa |
| Observed molecular weight | 58-61 kDa, 94-96 kDa |
| GenBank accession number | BC032376 |
| Gene Symbol | PHF17 |
| Gene ID (NCBI) | 79960 |
| RRID | AB_2165238 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IE81 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PHF17 was identified as a protein partner of the von Hippel-Lindau tumor suppressor pVHL. It is one component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It can act as a transcriptional coactivator that promote acetylation of nucleosomal histone H4 by KAT5. In addition, it ubiquitinates both phosphorylated and non-phosphorylated β-catenin and therefore regulates canonical Wnt signaling in both Wnt-off and Wnt-on phases. PHF17 exist several isoforms with molecular weight 100-110 and 55-65 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHF17 antibody 15032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Genome-wide association study and functional validation implicates JADE1 in tauopathy. | ||
J Biol Chem The ciliary protein nephrocystin-4 translocates the canonical Wnt regulator Jade-1 to the nucleus to negatively regulate β-catenin signaling. | ||
J Biol Chem Casein Kinase 1 Alpha Phosphorylates the Wnt-Regulator Jade-1 and Modulates its Activity. | ||
Cell Cycle Jade-1S phosphorylation induced by CK1α contributes to cell cycle progression. | ||
J Toxicol Sci Involvement of microRNA-4680-3p against phenytoin-induced cell proliferation inhibition in human palate cells | ||