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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHF17 Polyclonal antibody (28472-1-AP)

    WB analysis using 28472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PHF17 Polyclonal antibody (28472-1-AP)

    WB analysis of HepG2 using 28472-1-AP

    WB result of PHF17 antibody (28472-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF17 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using PHF17 Polyclonal antibody (28472-1-AP)

    WB analysis using 28472-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PHF17 Polyclonal antibody (28472-1-AP)

    WB analysis of A549 using 28472-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 28472-1-AP (PHF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHF17 Polyclonal antibody (28472-1-AP)

    IF Staining of HeLa using 28472-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF17 antibody (28472-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

PHF17 Polyclonal antibody

PHF17 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28472-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

FLJ22479, JADE1, KIAA1807, PHD finger protein 17, PHF17, Protein Jade 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, A549 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28472-1-AP targets PHF17 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHF17 fusion protein Ag29675 相同性解析による交差性が予測される生物種
Full Name PHD finger protein 17
Calculated molecular weight 96 kDa
Observed molecular weight65, 100-110 kDa
GenBank accession numberBC032376
Gene Symbol PHF17
Gene ID (NCBI) 79960
RRIDAB_2918168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IE81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PHF17, also named as JADE1 and KIAA1807, belongs to the JADE family. PHF17 codes for the Jade-1 protein. This protein interacts with histone acetyltransferase HBO1 and promotes histone acetylation in chromatin to regulate DNA replication and gene transcription. PHF17 is mostly expressed in the small intestine, prostate, mammary gland and kidney. PHF17 is involved in mitosis, meiosis and tumor suppression (PMID: 28134766). PHF17 has 3 isoforms with the molecular mass of 58 and 94-96 kDa. With modification, the MW of PHF17 may be migrated to about 65 and 100-110 kDa.

Protocols

Product Specific Protocols
WB protocol for PHF17 antibody 28472-1-APDownload protocol
IF protocol for PHF17 antibody 28472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols