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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHF20 Polyclonal antibody

PHF20 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22010-1-AP

Synonyms

C20orf104, GLEA2, Glioma expressed antigen 2, HCA58, Novel zinc finger protein, NZF, PHD finger protein 20, PHF20, Transcription factor TZP, TZP

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PHF20 Polyclonal antibody (22010-1-AP)

    WB analysis of HeLa using 22010-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PHF20 Polyclonal antibody (22010-1-AP)

    WB analysis of Jurkat using 22010-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PHF20 Polyclonal antibody (22010-1-AP)

    WB analysis of mouse testis using 22010-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PHF20 Polyclonal antibody (22010-1-AP)

    WB analysis of Jurkat using 22010-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHF20 Polyclonal antibody (22010-1-AP)

    IF Staining of HeLa using 22010-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22010-1-AP (PHF20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22010-1-AP is PHF20 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHF20 fusion protein Ag15686 相同性解析による交差性が予測される生物種
Full Name PHD finger protein 20
Calculated molecular weight 1012 aa, 115 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC150178
Gene symbol PHF20
Gene ID (NCBI) 51230
RRIDAB_2878970
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PHD finger protein 20 (PHF20),also known as NZF, TZP, GLEA2 or HCA58, is a 1,012 amino acid protein that localizes to the nucleus and contains one PHD-type zinc finger, one C2H2-type zinc finger and one A/T hook DNA-binding domain.PHF20 contains a tudor domain, which facilitates binding to di-methylated histone H4 Lys20. PHF20 is expressed in a variety of tissues, including liver, heart, lung, pancreas, spleen, testis and placenta, PHF20 is thought to function as a transcription factor, possibly playing a role in the development of cancers, including glioblastoma, lung cancer, colon cancer and ovarian cancer. PHF20 exists as three alternatively spliced isoforms (150kd,130kd and 120kd) which are encoded by a gene that maps to human chromosome 20 (22072714).

Protocols

Product Specific Protocols
WB protocol for PHF20 antibody 22010-1-APDownload protocol
IF protocol for PHF20 antibody 22010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Genet

Circular RNA circKIF2A Contributes to the Progression of Neuroblastoma Through Regulating PRPS1 Expression by Sponging miR-377-3p.

Authors - Quan Jin
View Article