Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25862-1-AP targets PHF23 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PHF23 fusion protein Ag23044 相同性解析による交差性が予測される生物種 |
| Full Name | PHD finger protein 23 |
| Calculated molecular weight | 403 aa, 44 kDa |
| Observed molecular weight | ~65 kDa |
| GenBank accession number | BC002509 |
| Gene Symbol | PHF23 |
| Gene ID (NCBI) | 79142 |
| RRID | AB_3085824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BUL5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PHD finger protein 23 (PHF23), also known as hJUNE-1b, belongs to the PHF23 family. As a putative epigenetic reader, the PHD domain of PHF23 specifically binds to tri-methylated lysine 4 of histone H3 (H3K4me3). PHF23 is widely expressed in human tissues and various cell lines and exerts a negative effect on autophagy by promoting the degradation of E3 ligase LRSAM1 (leucine-rich repeat and sterile alpha motif containing 1) (PMID: 32978226, 32380078). The observed molecular weight of PHF23 is ~65 kDa, which may be caused by post-translational modification. The specificity of this antibody was demonstrated by siRNA-mediated knockdown of target protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHF23 antibody 25862-1-AP | Download protocol |
| IF protocol for PHF23 antibody 25862-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |