Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
29516-1-AP targets PHF8 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PHF8 fusion protein Ag30701 相同性解析による交差性が予測される生物種 |
| Full Name | PHD finger protein 8 |
| Calculated molecular weight | 118 kDa |
| Observed molecular weight | 125-135 kDa |
| GenBank accession number | NM_001184896 |
| Gene Symbol | PHF8 |
| Gene ID (NCBI) | 23133 |
| RRID | AB_2935473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Plant homeodomain finger protein 8 (PHF8) is a JmjC domain-containing protein. PHF8 is localized in the nucleolus and may regulate rRNA transcription. As a member of the KDM7 subfamily of enzymes, PHF8 is involved in demethylation of lysine residues in histones such as H3K27me2/1, H3K9me2/1 and H4K20me1. PHF8 has been reported to participate in cancer development and metastasis of various types of tumors(PMID: 33111613). PHF8 has 5 isoforms produced by alternative splicing(Uniprot).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHF8 antibody 29516-1-AP | Download protocol |
| IHC protocol for PHF8 antibody 29516-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage |