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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHGDH Monoclonal antibody (67591-1-Ig)

    WB analysis using 67591-1-Ig (same clone as 67591-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67591-1-Ig (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PHGDH Monoclonal antibody (67591-1-Ig)

    IP experiment of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    IP result of anti-PHGDH (IP:67591-1-Ig, 4ug; Detection:67591-1-Ig 1:3000) with HeLa cells lysate 980 ug. This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using PHGDH Monoclonal antibody (67591-1-Ig)

    IHC staining of human urothelial carcinoma using 67591-1-Ig (same clone as 67591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67591-1-Ig (PHGDH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHGDH Monoclonal antibody (67591-1-Ig)

    IF Staining of HeLa using 67591-1-Ig (same clone as 67591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PHGDH antibody (67591-1-Ig, Clone: 1E8B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67591-1-PBS in a different storage buffer formulation.

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PHGDH Monoclonal antibody, PBS Only

PHGDH Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 67591-1-PBS
Clone No.1E8B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 3PGDH, 3 PGDH

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67591-1-PBS targets PHGDH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PHGDH fusion protein Ag6877 相同性解析による交差性が予測される生物種
Full Name phosphoglycerate dehydrogenase
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC000303
Gene Symbol PHGDH
Gene ID (NCBI) 26227
RRIDAB_2882799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43175
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).