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  • Featured Product
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PHGDH Recombinant antibody

PHGDH Recombinant Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5L16

Cat no : 81986-1-RR

Synonyms

3 PGDH, 3PGDH, PDG, PGAD, PGD, PGDH, PGDH3, PHGDH, phosphoglycerate dehydrogenase, SERA

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PHGDH Recombinant antibody (81986-1-RR)

    WB analysis of HEK-293 using 81986-1-RR

    WB result of PHGDH antibody (81986-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHGDH transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PHGDH Recombinant antibody (81986-1-RR)

    WB analysis using 81986-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81986-1-RR (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PHGDH Recombinant antibody (81986-1-RR)

    IP experiment of HeLa using 81986-1-RR

    IP result of anti-PHGDH (IP:81986-1-RR, 4ug; Detection:81986-1-RR 1:10000) with HeLa cells lysate 1760 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHGDH Recombinant antibody (81986-1-RR)

    IHC staining of human colon cancer using 81986-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81986-1-RR (PHGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHGDH Recombinant antibody (81986-1-RR)

    IHC staining of human colon cancer using 81986-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81986-1-RR (PHGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81986-1-RR

    Affinity and Kinetic Characterization of 81986-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81986-1-RR against Human PHGDH were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, mouse pancreas tissue, rat pancreas tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81986-1-RR targets PHGDH in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PHGDH fusion protein Ag6445 相同性解析による交差性が予測される生物種
Full Name phosphoglycerate dehydrogenase
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC000303
Gene symbol PHGDH
Gene ID (NCBI) 26227
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).

Protocols

Product Specific Protocols
WB protocol for PHGDH antibody 81986-1-RRDownload protocol
IHC protocol for PHGDH antibody 81986-1-RRDownload protocol
IP protocol for PHGDH antibody 81986-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols