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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHKA2 Polyclonal antibody

PHKA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24658-1-AP

Synonyms

GSD9A, PHK, PHKA2, PHKLA, PYK, PYKL, XLG, XLG2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHKA2 Polyclonal antibody (24658-1-AP)

    WB analysis using 24658-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24658-1-AP (PHKA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PHKA2 Polyclonal antibody (24658-1-AP)

    IHC staining of human liver using 24658-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24658-1-AP (PHKA2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PHKA2 Polyclonal antibody (24658-1-AP)

    IHC staining of human liver using 24658-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24658-1-AP (PHKA2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PHKA2 Polyclonal antibody (24658-1-AP)

    IF Staining of HepG2 using 24658-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PHKA2 antibody (24658-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24658-1-AP targets PHKA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHKA2 fusion protein Ag9473 相同性解析による交差性が予測される生物種
Full Name phosphorylase kinase, alpha 2 (liver)
Calculated molecular weight138 kDa
Observed molecular weight 138 kDa
GenBank accession numberBC014036
Gene symbol PHKA2
Gene ID (NCBI) 5256
RRIDAB_2879659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHKA2 antibody 24658-1-APDownload protocol
IHC protocol for PHKA2 antibody 24658-1-APDownload protocol
IF protocol for PHKA2 antibody 24658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Clin Invest

Imbalanced mitochondrial function provokes heterotaxy via aberrant ciliogenesis.

Authors - Martin D Burkhalter
View Article