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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHKG2 Recombinant antibody, PBS Only (Detector)

PHKG2 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240643E1

Cat no : 83663-2-PBS

Synonyms

PHK-gamma-T, PHK-gamma-LT, PHK gamma T, GSD9C, EC:2.7.11.19

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHKG2 Recombinant antibody (83663-2-RR)

    WB analysis using 83663-2-RR (same clone as 83663-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83663-2-RR (PHKG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PHKG2 Recombinant antibody (83663-2-RR)

    IF Staining of HEK-293 using 83663-2-RR (same clone as 83663-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PHKG2 antibody (83663-2-RR, Clone: 240643E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using PHKG2 Recombinant antibody (83663-2-RR)

    FC experiment of HEK-293T using 83663-2-RR (same clone as 83663-2-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug PHKG2 Recombinant antibody (83663-2-RR, Clone:240643E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PHKG2 Recombinant antibody (83663-2-RR)

    FC experiment of HepG2 using 83663-2-RR (same clone as 83663-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PHKG2 Recombinant antibody (83663-2-RR, Clone:240643E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83663-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00632-2

    Cytometric bead array standard curve of MP00632-2

    Cytometric bead array standard curve of MP00632-2, PHKG2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83663-4-PBS. Detection antibody: 83663-2-PBS. Standard: Ag6829. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83663-2-RR

    Affinity and Kinetic Characterization of 83663-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83663-2-RR against Human PHKG2 were performed. The affinity constant is 1.90 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83663-2-PBS targets PHKG2 as part of a matched antibody pair:

MP00632-2: 83663-4-PBS capture and 83663-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PHKG2 fusion protein Ag6829 相同性解析による交差性が予測される生物種
Full Name phosphorylase kinase, gamma 2 (testis)
Calculated molecular weight 46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC002541
Gene symbol PHKG2
Gene ID (NCBI) 5261
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa.