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- KD/KO Validated
PHLDA1 Polyclonal antibody
PHLDA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 18263-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, mouse brain tissue, BXPC-3 cells, HeLa cells, MDA-MB-231 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
18263-1-AP targets PHLDA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PHLDA1 fusion protein Ag13125 相同性解析による交差性が予測される生物種 |
| Full Name | pleckstrin homology-like domain, family A, member 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | BC018929 |
| Gene symbol | PHLDA1 |
| Gene ID (NCBI) | 22822 |
| RRID | AB_2163284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| IHC protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| IF protocol for PHLDA1 antibody 18263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Behav Immun PHLDA1 promotes microglia-mediated neuroinflammation via regulating K63-linked ubiquitination of TRAF6.
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Life Sci PHLDA1 is a new therapeutic target of oxidative stress and ischemia reperfusion-induced myocardial injury.
| ||
Inflammation TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT-GSK-3β Pathway.
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Hum Exp Toxicol Loss of PHLDA1 has a protective role in OGD/R-injured neurons via regulation of the GSK-3β/Nrf2 pathway.
| ||
Cell Death Dis The long non-coding RNA SNHG1 promotes glioma progression by competitively binding to miR-194 to regulate PHLDA1 expression.
| ||
Mol Ther Nucleic Acids lncRNA HIF1A Antisense RNA 2 Modulates Trophoblast Cell Invasion and Proliferation through Upregulating PHLDA1 Expression. |
