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  • Featured Product
  • KD/KO Validated

PHLDA1 Polyclonal antibody, PBS Only

PHLDA1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18263-1-PBS

Synonyms

Proline- and glutamine-rich protein, PQ-rich protein, Pleckstrin homology-like domain family A member 1, PHRIP, DT1P1B11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHLDA1 Polyclonal antibody (18263-1-AP)

    WB analysis using 18263-1-AP (same clone as 18263-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PHLDA1 Polyclonal antibody (18263-1-AP)

    WB analysis of mouse brain using 18263-1-AP (same clone as 18263-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18263-1-AP (PHLDA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PHLDA1 Polyclonal antibody (18263-1-AP)

    IHC staining of human skin cancer using 18263-1-AP (same clone as 18263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18263-1-AP (PHLDA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using PHLDA1 Polyclonal antibody (18263-1-AP)

    IF Staining of A375 using 18263-1-AP (same clone as 18263-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using PHLDA1 antibody (18263-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18263-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18263-1-PBS targets PHLDA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHLDA1 fusion protein Ag13125 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology-like domain, family A, member 1
Calculated molecular weight 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC018929
Gene symbol PHLDA1
Gene ID (NCBI) 22822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.