Validation Data Gallery

Filter: AllWBIHC

WB analysis of human placenta using 14661-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 14661-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14661-1-AP
WB result of PHLDA2 antibody (14661-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLDA2 transfected MCF-7 cells.
WB analysis of PC-3 using 14661-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 14661-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14661-1-AP (PHLDA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14661-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14661-1-AP (PHLDA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

PHLDA2 Polyclonal antibody

PHLDA2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14661-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

BRW1C, BWR1C, HLDA2, IPL, p17 BWR1C, PHLDA2, TSSC3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	L02 cells, human placenta tissue, MCF-7 cells, PC-3 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 2 publications below

Product Information

14661-1-AP targets PHLDA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PHLDA2 fusion protein Ag6331 相同性解析による交差性が予測される生物種
Full Name	pleckstrin homology-like domain, family A, member 2
Calculated molecular weight	17 kDa
Observed molecular weight	20-25 kDa
GenBank accession number	BC005034
Gene Symbol	PHLDA2
Gene ID (NCBI)	7262
RRID	AB_2163294
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q53GA4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PHLDA2 antibody 14661-1-AP	Download protocol
IHC protocol for PHLDA2 antibody 14661-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Nat Commun Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer. Authors - Yuji Kubota View Article
human	WB	Aging (Albany NY) PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway. Authors - Zhan Ma KD Validated View Article
human	WB	Pathol Res Pract FOSL1 transcriptionally regulates PHLDA2 to promote 5-FU resistance in colon cancer cells Authors - Guangyi Liu KD Validated View Article
Goat	WB	Gene Inhibition of PHLDA2 transcription by DNA methylation and YY1 in goat placenta. Authors - Ruihong Wang View Article
human	IHC,WB	Sci Rep Characteristics of lactate metabolism phenotype in hepatocellular carcinoma Authors - Jiacheng Zhang View Article
human	WB	Cell Metab PHLDA2-mediated phosphatidic acid peroxidation triggers a distinct ferroptotic response during tumor suppression Authors - Xin Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human placenta using 14661-1-AP

WB analysis using 14661-1-AP

WB analysis of MCF-7 using 14661-1-AP

WB analysis of PC-3 using 14661-1-AP

IHC staining of human prostate cancer using 14661-1-AP

IHC staining of human prostate cancer using 14661-1-AP

PHLDA2 Polyclonal antibody

PHLDA2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.

PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway.

FOSL1 transcriptionally regulates PHLDA2 to promote 5-FU resistance in colon cancer cells

Inhibition of PHLDA2 transcription by DNA methylation and YY1 in goat placenta.

Characteristics of lactate metabolism phenotype in hepatocellular carcinoma

PHLDA2-mediated phosphatidic acid peroxidation triggers a distinct ferroptotic response during tumor suppression