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KD/KO Validated

PHLDA2 Polyclonal antibody

Name: PHLDA2 Polyclonal antibody
Brand: Proteintech
SKU: 14661-1-AP

PHLDA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14661-1-AP

Synonyms

BRW1C, BWR1C, HLDA2, IPL, p17 BWR1C, PHLDA2, TSSC3

Validation Data Gallery

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AllWBIHC

WB analysis of human placenta using 14661-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 14661-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14661-1-AP
WB result of PHLDA2 antibody (14661-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLDA2 transfected MCF-7 cells.
WB analysis of PC-3 using 14661-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 14661-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14661-1-AP (PHLDA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14661-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14661-1-AP (PHLDA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 14661-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	L02 cells, human placenta tissue, MCF-7 cells, PC-3 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 2 publications below

Product Information

14661-1-AP targets PHLDA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PHLDA2 fusion protein Ag6331 相同性解析による交差性が予測される生物種
Full Name	pleckstrin homology-like domain, family A, member 2
Calculated molecular weight	17 kDa
Observed molecular weight	20-25 kDa
GenBank accession number	BC005034
Gene symbol	PHLDA2
Gene ID (NCBI)	7262
RRID	AB_2163294
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PHLDA2 antibody 14661-1-AP	Download protocol
IHC protocol for PHLDA2 antibody 14661-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Nat Commun Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer. Authors - Yuji Kubota View Article
human	WB	Aging (Albany NY) PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway. Authors - Zhan Ma KD Validated View Article
human	WB	Pathol Res Pract FOSL1 transcriptionally regulates PHLDA2 to promote 5-FU resistance in colon cancer cells Authors - Guangyi Liu KD Validated View Article
Goat	WB	Gene Inhibition of PHLDA2 transcription by DNA methylation and YY1 in goat placenta. Authors - Ruihong Wang View Article
human	IHC,WB	Sci Rep Characteristics of lactate metabolism phenotype in hepatocellular carcinoma Authors - Jiacheng Zhang View Article
human	WB	Cell Metab PHLDA2-mediated phosphatidic acid peroxidation triggers a distinct ferroptotic response during tumor suppression Authors - Xin Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHLDA2 Polyclonal antibody

PHLDA2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of human placenta using 14661-1-AP

WB analysis using 14661-1-AP

WB analysis of MCF-7 using 14661-1-AP

WB analysis of PC-3 using 14661-1-AP

IHC staining of human prostate cancer using 14661-1-AP

IHC staining of human prostate cancer using 14661-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.

PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway.

FOSL1 transcriptionally regulates PHLDA2 to promote 5-FU resistance in colon cancer cells

Inhibition of PHLDA2 transcription by DNA methylation and YY1 in goat placenta.

Characteristics of lactate metabolism phenotype in hepatocellular carcinoma

PHLDA2-mediated phosphatidic acid peroxidation triggers a distinct ferroptotic response during tumor suppression