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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PHLDB2 Polyclonal antibody (27940-1-AP)

    WB analysis of A431 using 27940-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27940-1-AP (PHLDB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human placenta using 27940-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human placenta using 27940-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of mouse heart using 27940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human skin cancer using 27940-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human skin cancer using 27940-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of human skin cancer using 27940-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PHLDB2 Polyclonal antibody (27940-1-AP)

    IHC staining of mouse heart using 27940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27940-1-AP (PHLDB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PHLDB2 Polyclonal antibody

PHLDB2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27940-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Protein LL5-beta, Pleckstrin homology-like domain family B member 2, LL5beta, LL5B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman skin cancer tissue, human placenta tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27940-1-AP targets PHLDB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHLDB2 fusion protein Ag27331 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology-like domain, family B, member 2
Calculated molecular weight 1253 aa, 142 kDa
Observed molecular weight150 kDa
GenBank accession numberBC142678
Gene Symbol PHLDB2
Gene ID (NCBI) 90102
RRIDAB_2935465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86SQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The pleckstrin homology-like domain (PHLD) class of proteins are multifunctional proteins comprised of two families of proteins, PHLDA and PHLDB, each with 3 members: PHLDA1, PHLDA2, PHDLA3, PHLDB1, PHLDB2, and PHLDB3 (PMID: 32429563). All the family members possess a pleckstrin homology (PH) domain. PHLB2, also known as LL5-beta, contains two predicted coiled-coil domains, a serine-rich region predicted to contain multiple protein kinase C phosphorylation sites in addition to a PH domain (PMID: 15851520). PHLB2 is a filamin-binding protein that is an effector of the PI3K signaling pathway (PMID: 17174070). It is a PtdIns(3,4,5)P3 (PIP3) binding protein and can translocate to the plasma membrane in a receptor and PI3K-dependant manner (PMID: 12376540). PHLB2 has also been reported as a component of the postsynaptic apparatus and a regulator of postsynaptic differentiation (PMID: 15851520).

Protocols

Product Specific Protocols
WB protocol for PHLDB2 antibody 27940-1-APDownload protocol
IHC protocol for PHLDB2 antibody 27940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols