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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHLPP Monoclonal antibody, PBS Only

PHLPP Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse , rat, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E8E6

Cat no : 67640-1-PBS

Synonyms

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PHLPP Monoclonal antibody (67640-1-Ig)

    WB analysis of mouse brain using 67640-1-Ig (same clone as 67640-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PHLPP Monoclonal antibody (67640-1-Ig)

    WB analysis of rat brain using 67640-1-Ig (same clone as 67640-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using PHLPP Monoclonal antibody (67640-1-Ig)

    WB analysis of pig brain using 67640-1-Ig (same clone as 67640-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PHLPP Monoclonal antibody (67640-1-Ig)

    WB analysis of HEK-293 using 67640-1-Ig (same clone as 67640-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67640-1-Ig (PHLPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67640-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67640-1-PBS targets PHLPP in WB, Indirect ELISA applications and shows reactivity with Human, mouse , rat, pig samples.

Tested Reactivity Human, mouse , rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PHLPP fusion protein Ag18982 相同性解析による交差性が予測される生物種
Full Name PH domain and leucine rich repeat protein phosphatase
Calculated molecular weight 1717 aa, 184 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC126277
Gene symbol PHLPP
Gene ID (NCBI) 23239
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.