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  • Featured Product
  • KD/KO Validated

PHLPPL Polyclonal antibody, PBS Only

PHLPPL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25244-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHLPPL Polyclonal antibody (25244-1-AP)

    WB analysis using 25244-1-AP (same clone as 25244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PHLPPL Polyclonal antibody (25244-1-AP)

    WB analysis of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    WB result of PHLPPL antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPPL transfected HeLa cells. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PHLPPL Polyclonal antibody (25244-1-AP)

    IP experiment of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    IP result of anti-PHLPPL (IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHLPPL Polyclonal antibody (25244-1-AP)

    IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PHLPPL Polyclonal antibody (25244-1-AP)

    IHC staining of human colon using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PHLPPL Polyclonal antibody (25244-1-AP)

    IHC staining of human colon using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHLPPL Polyclonal antibody (25244-1-AP)

    IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHLPPL Polyclonal antibody (25244-1-AP)

    IF Staining of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPPL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25244-1-PBS targets PHLPPL in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHLPPL fusion protein Ag19000 相同性解析による交差性が予測される生物種
Full Name PH domain and leucine rich repeat protein phosphatase-like
Calculated molecular weight 1323 aa, 147 kDa
Observed molecular weight 147 kDa
GenBank accession numberBC129927
Gene symbol PHLPPL
Gene ID (NCBI) 23035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PHLPPL also named as KIAA0931 or PHLPP2 is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.