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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHLPPL Polyclonal antibody (25244-1-AP)

    WB analysis using 25244-1-AP (same clone as 25244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PHLPPL Polyclonal antibody (25244-1-AP)

    WB analysis of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    WB result of PHLPPL antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPPL transfected HeLa cells. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PHLPPL Polyclonal antibody (25244-1-AP)

    IP experiment of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    IP result of anti-PHLPPL (IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHLPPL Polyclonal antibody (25244-1-AP)

    IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PHLPPL Polyclonal antibody (25244-1-AP)

    IHC staining of human colon using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PHLPPL Polyclonal antibody (25244-1-AP)

    IHC staining of human colon using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PHLPPL Polyclonal antibody (25244-1-AP)

    IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHLPPL Polyclonal antibody (25244-1-AP)

    IF Staining of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPPL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25244-1-PBS targets PHLPPL in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHLPPL fusion protein Ag19000 相同性解析による交差性が予測される生物種
Full Name PH domain and leucine rich repeat protein phosphatase-like
Calculated molecular weight 1323 aa, 147 kDa
Observed molecular weight 147 kDa
GenBank accession numberBC129927
Gene Symbol PHLPPL
Gene ID (NCBI) 23035
RRIDAB_2879985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZVD8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PHLPPL also named as KIAA0931 or PHLPP2 is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.