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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHOSPHO2 Polyclonal antibody

PHOSPHO2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11869-1-AP

Synonyms

EC:3.1.3.74

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHOSPHO2 Polyclonal antibody (11869-1-AP)

    WB analysis using 11869-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11869-1-AP (PHOSPHO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PHOSPHO2 Polyclonal antibody (11869-1-AP)

    IHC staining of mouse testis using 11869-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11869-1-AP (PHOSPHO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PHOSPHO2 Polyclonal antibody (11869-1-AP)

    IHC staining of mouse testis using 11869-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11869-1-AP (PHOSPHO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11869-1-AP targets PHOSPHO2 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHOSPHO2 fusion protein Ag2451 相同性解析による交差性が予測される生物種
Full Name phosphatase, orphan 2
Calculated molecular weight 241 aa, 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC022324
Gene symbol PHOSPHO2
Gene ID (NCBI) 493911
RRIDAB_3085383
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PHOSPHO2 belongs to the HAD-like hydrolase superfamily and PHOSPHO family. PHOSPHO2 is a phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). PHOSPHO2 is also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate. The molecular weight of PHOSPHO2 is 28 kDa.

Protocols

Product Specific Protocols
WB protocol for PHOSPHO2 antibody 11869-1-APDownload protocol
IHC protocol for PHOSPHO2 antibody 11869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols