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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHOSPHO2 Polyclonal antibody

PHOSPHO2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11869-1-AP

Synonyms

EC:3.1.3.74

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHOSPHO2 Polyclonal antibody (11869-1-AP)

    WB analysis using 11869-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11869-1-AP (PHOSPHO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PHOSPHO2 Polyclonal antibody (11869-1-AP)

    IHC staining of mouse testis using 11869-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11869-1-AP (PHOSPHO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PHOSPHO2 Polyclonal antibody (11869-1-AP)

    IHC staining of mouse testis using 11869-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11869-1-AP (PHOSPHO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11869-1-AP targets PHOSPHO2 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHOSPHO2 fusion protein Ag2451 相同性解析による交差性が予測される生物種
Full Name phosphatase, orphan 2
Calculated molecular weight 241 aa, 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC022324
Gene symbol PHOSPHO2
Gene ID (NCBI) 493911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PHOSPHO2 belongs to the HAD-like hydrolase superfamily and PHOSPHO family. PHOSPHO2 is a phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). PHOSPHO2 is also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate. The molecular weight of PHOSPHO2 is 28 kDa.

Protocols

Product Specific Protocols
WB protocol for PHOSPHO2 antibody 11869-1-APDownload protocol
IHC protocol for PHOSPHO2 antibody 11869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols