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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using PHYH Polyclonal antibody (12858-1-AP)

    WB analysis of human liver using 12858-1-AP (same clone as 12858-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using PHYH Polyclonal antibody (12858-1-AP)

    WB analysis of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PHYH Polyclonal antibody (12858-1-AP)

    WB analysis of Jurkat using 12858-1-AP (same clone as 12858-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PHYH Polyclonal antibody (12858-1-AP)

    WB analysis of HEK-293 using 12858-1-AP (same clone as 12858-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PHYH Polyclonal antibody (12858-1-AP)

    IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PHYH Polyclonal antibody (12858-1-AP)

    IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

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PHYH Polyclonal antibody, PBS Only

PHYH Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12858-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12858-1-PBS targets PHYH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHYH fusion protein Ag3569 相同性解析による交差性が予測される生物種
Full Name phytanoyl-CoA 2-hydroxylase
Calculated molecular weight 39 kDa
Observed molecular weight 36 kDa, 70 kDa
GenBank accession numberBC029512
Gene Symbol PHYH
Gene ID (NCBI) 5264
RRIDAB_2163745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14832
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PHYH (Phytanoyl-CoA 2-hydroxylase), also known as Phytanic acid oxidase or Phytanoyl-CoA alpha-hydroxylase, is a peroxisomal enzyme which converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. It catalyzes the first step in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. Mutations in this gene can cause Refsum disease (RD) and deficient protein activity can cause Zellweger syndrome and rhizomelic chondrodysplasia punctata. This antibody (12858-1-AP) detects two bands: 36kd and 70kd. The 70kd band may probably be the dimeric form of PHYH.