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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHYH Polyclonal antibody, PBS Only

PHYH Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12858-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using PHYH Polyclonal antibody (12858-1-AP)

    WB analysis of human liver using 12858-1-AP (same clone as 12858-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using PHYH Polyclonal antibody (12858-1-AP)

    WB analysis of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PHYH Polyclonal antibody (12858-1-AP)

    WB analysis of Jurkat using 12858-1-AP (same clone as 12858-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PHYH Polyclonal antibody (12858-1-AP)

    WB analysis of HEK-293 using 12858-1-AP (same clone as 12858-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12858-1-AP (PHYH antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PHYH Polyclonal antibody (12858-1-AP)

    IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PHYH Polyclonal antibody (12858-1-AP)

    IHC staining of human kidney using 12858-1-AP (same clone as 12858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 12858-1-AP (PHYH antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12858-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12858-1-PBS targets PHYH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHYH fusion protein Ag3569 相同性解析による交差性が予測される生物種
Full Name phytanoyl-CoA 2-hydroxylase
Calculated molecular weight 39 kDa
Observed molecular weight 36 kDa, 70 kDa
GenBank accession numberBC029512
Gene symbol PHYH
Gene ID (NCBI) 5264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PHYH (Phytanoyl-CoA 2-hydroxylase), also known as Phytanic acid oxidase or Phytanoyl-CoA alpha-hydroxylase, is a peroxisomal enzyme which converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. It catalyzes the first step in the alpha-oxidation of phytanic acid, a branched-chain fatty acid. Mutations in this gene can cause Refsum disease (RD) and deficient protein activity can cause Zellweger syndrome and rhizomelic chondrodysplasia punctata. This antibody (12858-1-AP) detects two bands: 36kd and 70kd. The 70kd band may probably be the dimeric form of PHYH.