Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse brain tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16937-1-AP targets PHYHIPL in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PHYHIPL fusion protein Ag10435 相同性解析による交差性が予測される生物種 |
| Full Name | phytanoyl-CoA 2-hydroxylase interacting protein-like |
| Calculated molecular weight | 376 aa, 43 kDa |
| Observed molecular weight | 39 kDa and 42-45 kDa |
| GenBank accession number | BC011268 |
| Gene Symbol | PHYHIPL |
| Gene ID (NCBI) | 84457 |
| RRID | AB_2163761 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96FC7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PHYHIPL, a kind of mitochondrial protein, is associated with energy production, oxidative stress and organelle dynamics. It is reported that global ischemia is related to the change of PHYHIPL protein level. Compared with the control group, the level of APOE (may be involved in abnormal regulation of mitochondrial function) is significantly increased after ischemic stimulation, while the APOE genotype has a effect on PHYHIPL protein level. 16937-1-AP detects 39 kDa isoform and 42-45 kDa isoform in SDS-PAGE. (PMID: 21878944)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHYHIPL antibody 16937-1-AP | Download protocol |
| IHC protocol for PHYHIPL antibody 16937-1-AP | Download protocol |
| IF protocol for PHYHIPL antibody 16937-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |