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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

PI16 Polyclonal antibody

PI16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12267-1-AP

Synonyms

CRISP 9, CRISP9, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI16 Polyclonal antibody (12267-1-AP)

    WB analysis using 12267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using PI16 Polyclonal antibody (12267-1-AP)

    WB analysis of A2780 using 12267-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PI16 Polyclonal antibody (12267-1-AP)

    WB analysis of COLO 320 using 12267-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12267-1-AP (PI16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of human liver cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of human liver cancer using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of mouse testis using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PI16 Polyclonal antibody (12267-1-AP)

    IHC staining of mouse testis using 12267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12267-1-AP (PI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSKOV-3 cells, A2780 cells, COLO 320 cells, A549 cells
Positive IHC detected inhuman liver cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12267-1-AP targets PI16 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI16 fusion protein Ag2925 相同性解析による交差性が予測される生物種
Full Name peptidase inhibitor 16
Calculated molecular weight 463 aa, 49 kDa
Observed molecular weight49 kDa
GenBank accession numberBC022399
Gene symbol PI16
Gene ID (NCBI) 221476
RRIDAB_10697651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PI16 antibody 12267-1-APDownload protocol
IHC protocol for PI16 antibody 12267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIHC,WB,CoIP

Cell Mol Biol Lett

Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO

Authors - Xiangqin Kuang
  • KD Validated
View Article