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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PI16 Polyclonal antibody

PI16 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30527-1-AP

Synonyms

CRISP 9, CRISP9, DKFZp586B1817, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI16 Polyclonal antibody (30527-1-AP)

    WB analysis using 30527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PI16 Polyclonal antibody (30527-1-AP)

    WB analysis using 30527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using PI16 Polyclonal antibody (30527-1-AP)

    IF Staining of SKOV-3 using 30527-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PI16 antibody (30527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inCOLO 320 cells, rat heart tissue, SKOV-3 cells
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30527-1-AP targets PI16 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI16 fusion protein Ag32556 相同性解析による交差性が予測される生物種
Full Name peptidase inhibitor 16
Calculated molecular weight 463 aa, 49 kDa
Observed molecular weight 100 kDa, 49 kDa
GenBank accession numberBC022399
Gene symbol PI16
Gene ID (NCBI) 221476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PI16 antibody 30527-1-APDownload protocol
IF protocol for PI16 antibody 30527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols