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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    WB analysis using 67121-1-Ig (same clone as 67121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    WB analysis of HeLa using 67121-1-Ig (same clone as 67121-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    WB analysis of HEK-293 using 67121-1-Ig (same clone as 67121-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    WB analysis of MCF-7 using 67121-1-Ig (same clone as 67121-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IHC staining of human lung cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IHC staining of human lung cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IHC staining of human colon cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IHC staining of human colon cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IHC staining of human cervical cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IHC staining of human cervical cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IHC staining of human colon cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IHC staining of human colon cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PI3 Kinase p110 Beta Monoclonal antibody (67121-1-Ig)

    IF Staining of HeLa using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p110 Beta antibody (67121-1-Ig, Clone: 1B2A1 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

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PI3 Kinase p110 Beta Monoclonal antibody, PBS Only

PI3 Kinase p110 Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67121-1-PBS
Clone No.1B2A1

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PI3K p110(beta), PIK3CB, PI3K, PI3 kinase subunit beta, p110 BETA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67121-1-PBS targets PI3 Kinase p110 Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p110 Beta fusion protein Ag17505 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, beta polypeptide
Calculated molecular weight 1070 aa, 123 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC114432
Gene Symbol PI3 Kinase p110 Beta
Gene ID (NCBI) 5291
RRIDAB_2882424
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42338
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).