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  • Featured Product
  • KD/KO Validated

PI3 Kinase p55 Gamma Polyclonal antibody, PBS Only

PI3 Kinase p55 Gamma Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27035-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    WB analysis of MCF-7 using 27035-1-AP (same clone as 27035-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27035-1-AP (PI 3 Kinase p55 gamma antibody at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    WB analysis of HEK-293 using 27035-1-AP (same clone as 27035-1-PBS)

    WB result of PI 3 Kinase p55 gamma antibody (27035-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI 3 Kinase p55 gamma transfected HEK-293 cells. This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    WB analysis of HUVEC using 27035-1-AP (same clone as 27035-1-PBS)

    WB result of PIK3R3 antibody (27035-1-AP) on whole cell extract from HUVEC targeted by shRNAs (#1, #2) or a control shRNA (NT). (Data from Dr. Fuqiang Geng, Weill Cornell Medical College). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    IHC staining of human kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27035-1-AP (PI 3 Kinase p55 gamma antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    IHC staining of mouse testis using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PI3 Kinase p55 Gamma Polyclonal antibody (27035-1-AP)

    IHC staining of mouse kidney using 27035-1-AP (same clone as 27035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27035-1-AP (PI3 Kinase p55 Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27035-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27035-1-PBS targets PI3 Kinase p55 Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI3 Kinase p55 Gamma fusion protein Ag25884 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Calculated molecular weight 85 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC021622
Gene symbol PI3 Kinase p55 Gamma
Gene ID (NCBI) 8503
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.