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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Beta Monoclonal antibody (67644-1-Ig)

    WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PI3 Kinase p85 Beta antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Beta Monoclonal antibody (67644-1-Ig)

    WB analysis using 67644-1-Ig (same clone as 67644-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67644-1-Ig (PIK3R2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PI3 Kinase p85 Beta Monoclonal antibody (67644-1-Ig)

    IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PI3 Kinase p85 Beta Monoclonal antibody (67644-1-Ig)

    IHC staining of human colon using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67644-1-Ig (PIK3R2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PI3 Kinase p85 Beta Monoclonal antibody (67644-1-Ig)

    IF Staining of HeLa using 67644-1-Ig (same clone as 67644-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p85 Beta antibody (67644-1-Ig, Clone: 2B3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67644-1-PBS in a different storage buffer formulation.

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PI3 Kinase p85 Beta Monoclonal antibody, PBS Only

PI3 Kinase p85 Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67644-1-PBS
Clone No.2B3B6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K regulatory subunit beta, PIK3R2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67644-1-PBS targets PI3 Kinase p85 Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p85 Beta fusion protein Ag14369 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Calculated molecular weight 728 aa, 82 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC014170
Gene Symbol PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
RRIDAB_2882844
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00459
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).