Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 15 publications below |
Product Information
27921-1-AP targets PI3 Kinase p110 Alpha in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PI3 Kinase p110 Alpha fusion protein Ag27539 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoinositide-3-kinase, catalytic, alpha polypeptide |
| Calculated molecular weight | 1068 aa, 124 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC113601 |
| Gene Symbol | PIK3CA |
| Gene ID (NCBI) | 5290 |
| RRID | AB_2918135 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42336 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p110 Alpha antibody 27921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology. | ||
Heliyon Anticancer potential of grifolin in lung cancer treatment through PI3K/AKT pathway inhibition | ||
JCI Insight Regulatory T-cell and neutrophil extracellular traps interaction contributes to the development of immunosuppression in sepsis | ||
Anticancer Drugs STK214947, a novel indole alkaloids, inhibits HeLa and SK-HEP-1 cells survival and EMT process by blocking the Notch3 and Akt signals | ||
J Immunother Vitexin Inhibits TNBC Progression and Metastasis by Modulating Macrophage Polarization Through EGFR Signaling | ||
Eur J Pharmacol Novel Applications of Metformin in the Treatment of Septic Myocardial Injury Based on Metabolomics and Network Pharmacology |