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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI3 Kinase p110 Delta Polyclonal antibody (21708-1-AP)

    WB analysis using 21708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21708-1-AP (PI3 Kinase p110 Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using PI3 Kinase p110 Delta Polyclonal antibody (21708-1-AP)

    IF Staining of LNCaP using 21708-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using PI3 Kinase p110 Delta antibody (21708-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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PI3 Kinase p110 Delta Polyclonal antibody

PI3 Kinase p110 Delta Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21708-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

PI3K p110(delta), PIK3CD, PI3K delta, PI3 kinase subunit delta, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, Raji cells, RAW 264.7 cells
Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21708-1-AP targets PI3 Kinase p110 Delta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI3 Kinase p110 Delta fusion protein Ag16326 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, delta polypeptide
Calculated molecular weight 1044 aa, 120 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberBC132919
Gene Symbol PIK3CD
Gene ID (NCBI) 5293
RRIDAB_3085666
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00329
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Delta antibody 21708-1-APDownload protocol
IF protocol for PI3 Kinase p110 Delta antibody 21708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Mol Life Sci

FAM76B regulates PI3K/Akt/NF-κB-mediated M1 macrophage polarization by influencing the stability of PIK3CD mRNA

Authors - Juan Wang
View Article
ratIF

J Ethnopharmacol

Uncovering the mechanisms of Zhubi decoction against rheumatoid arthritis through an integrated study of network pharmacology, metabolomics, and intestinal flora

Authors - Jing Liu
View Article