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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PI3 Kinase p85 Beta Polyclonal antibody

PI3 Kinase p85 Beta Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25868-1-AP

Synonyms

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K p85(beta), PI3K regulatory subunit beta, PIK3R2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    WB analysis using 25868-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    WB analysis using 25868-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    IHC staining of human placenta using 25868-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    IHC staining of mouse kidney using 25868-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    IHC staining of mouse small intestine using 25868-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    IHC staining of rat kidney using 25868-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    IHC staining of rat kidney using 25868-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    IHC staining of rat small intestine using 25868-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using PI3 Kinase p85 Beta Polyclonal antibody (25868-1-AP)

    IHC staining of rat small intestine using 25868-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, U-937 cells
Positive IHC detected inhuman placenta tissue, mouse kidney tissue, mouse small intestine tissue, rat kidney tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25868-1-AP targets PI3 Kinase p85 Beta in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI3 Kinase p85 Beta fusion protein Ag14369 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Calculated molecular weight 728 aa, 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC014170
Gene symbol PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p85 Beta antibody 25868-1-APDownload protocol
IHC protocol for PI3 Kinase p85 Beta antibody 25868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols