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  • Featured Product
  • KD/KO Validated

PI4K2A Polyclonal antibody

PI4K2A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15318-1-AP

Synonyms

PIK42A, PI4KII, PI 4-kinase II alpha, Phosphatidylinositol 4-kinase type II-alpha, Phosphatidylinositol 4-kinase type 2-alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI4K2A Polyclonal antibody (15318-1-AP)

    WB analysis using 15318-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15318-1-AP (PI4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PI4K2A Polyclonal antibody (15318-1-AP)

    WB analysis of K-562 using 15318-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15318-1-AP (PI4K2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PI4K2A Polyclonal antibody (15318-1-AP)

    WB analysis using 15318-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15318-1-AP (PI4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15318-1-AP targets PI4K2A in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI4K2A fusion protein Ag7564 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol 4-kinase type 2 alpha
Calculated molecular weight 54 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC003167
Gene symbol PI4K2A
Gene ID (NCBI) 55361
RRIDAB_2268225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PI4K2A antibody 15318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
  • KD Validated
View Article
humanIF

EMBO J

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Authors - Zhengming Wu
View Article
humanWB

Proc Natl Acad Sci U S A

Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.

Authors - Lei Shi
View Article
humanWB

Nat Biotechnol

A palmitoyl transferase chemical-genetic system to map ZDHHC-specific S-acylation

Authors - Cory A Ocasio
View Article
humanWB

Mol Cell

Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH

Authors - Pinki Gahlot
  • KD Validated
View Article