Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
23395-1-AP targets PIAS1 in WB, IHC, IF, IP, coIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PIAS1 fusion protein Ag18132 相同性解析による交差性が予測される生物種 |
| Full Name | protein inhibitor of activated STAT, 1 |
| Calculated molecular weight | 651 aa, 72 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC121797 |
| Gene Symbol | PIAS1 |
| Gene ID (NCBI) | 8554 |
| RRID | AB_2879272 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75925 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PIAS1 antibody 23395-1-AP | Download protocol |
| IHC protocol for PIAS1 antibody 23395-1-AP | Download protocol |
| IP protocol for PIAS1 antibody 23395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun CryoET reveals organelle phenotypes in huntington disease patient iPSC-derived and mouse primary neurons | ||
Nat Commun Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin | ||
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
PLoS Pathog PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.
| ||
Mol Med Rep Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose | ||
DNA Cell Biol miR-182-5p and miR-96-5p Target PIAS1 and Mediate the Negative Feedback Regulatory Loop between PIAS1 and STAT3 in Endometrial Cancer. |