☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

PIAS1 Polyclonal antibody

PIAS1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 23395-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, coIP, ELISA

DDXBP1, DEAD/H box binding protein 1, E3 SUMO protein ligase PIAS1, Gu binding protein, GU/RH II, PIAS1, ZMIZ3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23395-1-AP targets PIAS1 in WB, IHC, IF, IP, coIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIAS1 fusion protein Ag18132 相同性解析による交差性が予測される生物種
Full Name protein inhibitor of activated STAT, 1
Calculated molecular weight 651 aa, 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC121797
Gene Symbol PIAS1
Gene ID (NCBI) 8554
RRIDAB_2879272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PIAS1 antibody 23395-1-APDownload protocol
IHC protocol for PIAS1 antibody 23395-1-APDownload protocol
IP protocol for PIAS1 antibody 23395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

CryoET reveals organelle phenotypes in huntington disease patient iPSC-derived and mouse primary neurons

Authors - Gong-Her Wu
humanWB

Nat Commun

Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin

Authors - Tao Zhang
mouse,ratWB,coIP

J Hazard Mater

Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.

Authors - Junrong Wu
humanWB,IF

PLoS Pathog

PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.

Authors - Guangwen Wang
  • KD Validated
humanWB

Mol Med Rep

Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose

Authors - Cunyang Gu
humanIHC, WB

DNA Cell Biol

miR-182-5p and miR-96-5p Target PIAS1 and Mediate the Negative Feedback Regulatory Loop between PIAS1 and STAT3 in Endometrial Cancer.

Authors - Yuzhen Xiao
Loading...