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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIAS1 Recombinant antibody, PBS Only

PIAS1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C22

Cat no : 82873-2-PBS

Synonyms

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIAS1 Recombinant antibody (82873-2-RR)

    WB analysis using 82873-2-RR (same clone as 82873-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82873-2-RR (PIAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PIAS1 Recombinant antibody (82873-2-RR)

    IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PIAS1 Recombinant antibody (82873-2-RR)

    IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PIAS1 Recombinant antibody (82873-2-RR)

    IF Staining of HepG2 using 82873-2-RR (same clone as 82873-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIAS1 antibody (82873-2-RR, Clone: 1C22 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82873-2-PBS

    Affinity and Kinetic Characterization of 82873-2-PBS

    Biolayer interferometry (BLl) kinetic assays of 82873-2-RR against Human PIAS1 were performed. The affinity constant is 5.01 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82873-2-PBS targets PIAS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PIAS1 fusion protein Ag18132 相同性解析による交差性が予測される生物種
Full Name protein inhibitor of activated STAT, 1
Calculated molecular weight 651 aa, 72 kDa
Observed molecular weight72 kDa
GenBank accession numberBC121797
Gene symbol PIAS1
Gene ID (NCBI) 8554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.