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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIAS1 Recombinant antibody (82873-2-RR)

    WB analysis using 82873-2-RR (same clone as 82873-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82873-2-RR (PIAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PIAS1 Recombinant antibody (82873-2-RR)

    IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PIAS1 Recombinant antibody (82873-2-RR)

    IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PIAS1 Recombinant antibody (82873-2-RR)

    IF Staining of HepG2 using 82873-2-RR (same clone as 82873-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIAS1 antibody (82873-2-RR, Clone: 1C22 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PIAS1 Recombinant antibody (82873-2-RR)

    FC experiment of HeLa using 82873-2-RR (same clone as 82873-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PIAS1 Recombinant antibody (82873-2-RR, Clone:1C22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82873-2-RR

    Affinity and Kinetic Characterization of 82873-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82873-2-RR against Human PIAS1 were performed. The affinity constant is 5.01 nM.

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PIAS1 Recombinant antibody, PBS Only

PIAS1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82873-2-PBS
Clone No.1C22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82873-2-PBS targets PIAS1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PIAS1 fusion protein Ag18132 相同性解析による交差性が予測される生物種
Full Name protein inhibitor of activated STAT, 1
Calculated molecular weight 651 aa, 72 kDa
Observed molecular weight72 kDa
GenBank accession numberBC121797
Gene Symbol PIAS1
Gene ID (NCBI) 8554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.