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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using PIAS4 Polyclonal antibody (18469-1-AP)

    WB analysis of HL-60 using 18469-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PIAS4 Polyclonal antibody (18469-1-AP)

    WB analysis of K-562 using 18469-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PIAS4 Polyclonal antibody (18469-1-AP)

    WB analysis of Jurkat using 18469-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18469-1-AP (PIAS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PIAS4 Polyclonal antibody (18469-1-AP)

    IF Staining of HeLa using 18469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIAS4 antibody (18469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using PIAS4 Polyclonal antibody (18469-1-AP)

    FC experiment of HeLa using 18469-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PIAS4 (18469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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PIAS4 Polyclonal antibody

PIAS4 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 18469-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PIASy, PIAS-gamma, PIASG, PIAS gamma, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18469-1-AP targets PIAS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIAS4 fusion protein Ag13316 相同性解析による交差性が予測される生物種
Full Name protein inhibitor of activated STAT, 4
Calculated molecular weight 57 kDa
Observed molecular weight 57-70 kDa
GenBank accession numberBC010047
Gene Symbol PIAS4
Gene ID (NCBI) 51588
RRIDAB_2164380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N2W9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members(PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).

Protocols

Product Specific Protocols
WB protocol for PIAS4 antibody 18469-1-APDownload protocol
IF protocol for PIAS4 antibody 18469-1-APDownload protocol
FC protocol for PIAS4 antibody 18469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols