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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PICALM Polyclonal antibody

PICALM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28554-1-AP

Synonyms

CALM, CLTH, LAP, PICALM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PICALM Polyclonal antibody (28554-1-AP)

    WB analysis using 28554-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28554-1-AP (PICALM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PICALM Polyclonal antibody (28554-1-AP)

    IHC staining of mouse brain using 28554-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28554-1-AP (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28554-1-AP targets PICALM in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PICALM fusion protein Ag29505 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol binding clathrin assembly protein
Calculated molecular weight 71 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC064357
Gene symbol PICALM
Gene ID (NCBI) 8301
RRIDAB_2918175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

Protocols

Product Specific Protocols
WB protocol for PICALM antibody 28554-1-APDownload protocol
IHC protocol for PICALM antibody 28554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols