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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PICALM Monoclonal antibody

PICALM Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F6G7

Cat no : CL488-67564

Synonyms

Phosphatidylinositol-binding clathrin assembly protein, LAP, CLTH, CALM, 1F6G7

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PICALM Monoclonal an (CL488-67564)

    IF Staining of HeLa using CL488-67564

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PICALM antibody (CL488-67564, Clone: 1F6G7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated PICALM Monoclonal an (CL488-67564)

    FC experiment of HEK-293 using CL488-67564

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PICALM (CL488-67564, Clone:1F6G7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67564 targets PICALM in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PICALM fusion protein Ag29512 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol binding clathrin assembly protein
Calculated molecular weight 71 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC064357
Gene symbol PICALM
Gene ID (NCBI) 8301
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PICALM antibody CL488-67564Download protocol
FC protocol for CL Plus 488 PICALM antibody CL488-67564Download protocol
Standard Protocols
Click here to view our Standard Protocols