PIF1 Polyclonal antibody
PIF1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 19006-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
19006-1-AP targets PIF1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PIF1 fusion protein Ag13522 相同性解析による交差性が予測される生物種 |
| Full Name | PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 63-73 kDa |
| GenBank accession number | BC018978 |
| Gene symbol | PIF1 |
| Gene ID (NCBI) | 80119 |
| RRID | AB_10598312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIF1, also named as ATP-dependent DNA helicase PIF1, is a 641 amino acid protein, which belongs to the helicase family. PIF1 as a DNA-dependent ATPase and 5'-3' DNA helicase is required for the maintenance of both mitochondrial and nuclear genome stability. PIF1 localizes in the nucleus and is involved in the maintenance of telomeric DNA. PIF1 exists as four isoforms. isoform 1 is a 70 kDa protein and isoform 2 is 63 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PIF1 antibody 19006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis CircNEIL3 mediates pyroptosis to influence lung adenocarcinoma radiotherapy by upregulating PIF1 through miR-1184 inhibition. |
