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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIF1 Polyclonal antibody, PBS Only

PIF1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19006-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using PIF1 Polyclonal antibody (19006-1-AP)

    WB analysis of mouse thymus using 19006-1-AP (same clone as 19006-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19006-1-AP (PIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19006-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using PIF1 Polyclonal antibody (19006-1-AP)

    WB analysis of mouse thymus using 19006-1-AP (same clone as 19006-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19006-1-AP (PIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19006-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19006-1-PBS targets PIF1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIF1 fusion protein Ag13522 相同性解析による交差性が予測される生物種
Full Name PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae)
Calculated molecular weight 70 kDa
Observed molecular weight 63-73 kDa
GenBank accession numberBC018978
Gene symbol PIF1
Gene ID (NCBI) 80119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIF1, also named as ATP-dependent DNA helicase PIF1, is a 641 amino acid protein, which belongs to the helicase family. PIF1 as a DNA-dependent ATPase and 5'-3' DNA helicase is required for the maintenance of both mitochondrial and nuclear genome stability. PIF1 localizes in the nucleus and is involved in the maintenance of telomeric DNA. PIF1 exists as four isoforms. isoform 1 is a 70 kDa protein and isoform 2 is 63 kDa protein.