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PIGA Polyclonal antibody

PIGA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13679-1-AP

Synonyms

GlcNAc PI synthesis protein, GPI3, PIG A, PIGA


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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inmouse lung tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13679-1-AP targets PIGA in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIGA fusion protein Ag4530 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol glycan anchor biosynthesis, class A
Calculated molecular weight 484 aa, 54 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC038236
Gene symbol PIGA
Gene ID (NCBI) 5277
RRIDAB_2164656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PIGA antibody 13679-1-APDownload protocol
IHC protocol for PIGA antibody 13679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

Hum Mol Genet

CNS glycosylphosphatidylinositol deficiency results in delayed white matter development, ataxia and premature death in a novel mouse model.

Authors - Marshall Lukacs
  • KO Validated